Medical Inspection – Health Blog

Histology and immunohistochemistry

Three wounds on the back of each rat were sequentially removed from the site at days 3, 7 and 14 after surgery. Each specimen was divided into two equal parts. One half of the specimens were fixed in 4% paraformaldehyde, washed in tap water, dehydrated in a series of graded ethanol solutions, cleared in xylene, and then embedded in paraffin for light microscopic examination. The sections (4 pm thickness) were then mounted on glass slides, hydrated with distilled water, and subjected to hematoxylin-eosin staining (Vector Laboratories, Burlingame, CA, USA).

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Adenoviral vectors

Recombinant adenoviruses that expressed COMP- Ang 1 or LacZ were constructed using the pAdEasy vector system (Ade-COMP-Ang 1 and Ade-LacZ) (Ade-COMP-Ang 1 and Ade-LacZ were generous gifts from Prof. Gou Young Koh at Korea Advanced Institute of Science and Technology, Daejeon, Korea).

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INTRODUCTION

The closure of cutaneous wounds involves events such as hemorrhage, inflammation, re-epithelializa- tion, granulation tissue formation, and the late remodeling phase of tissue repair. The early and acute phases of repair involve macrophage ac­cumulation, fibroblast ingrowth, matrix deposition, and angiogenesis. These events are triggered by a complex mixture of cytokines and growth factors that are released at the site of injury.

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The review of correlation between pilosebaceous cyst and apocrine hidrocystoma is limited. But Anderson et al presented a combination of apocrine hidrocystoma and epidermal inclusion cyst. He reported four unusual cutaneous cysts in which apocrine epithelium is juxtaposed with keratinizing squamous epithelium and suggested that these cysts arise at the junction of glandular and keratinizing squamous epithelium, accounting for the mixture of lining cells seen histologically. It is also possible that squamous metaplasia within an apocrine hidrocys- toma accounts for the mixture of lining epithelium. Finally, he suggested these cysts represent collision lesions in which an apocrine hidrocystoma has fused with an epidermal inclusion cyst.

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Milia is a small dermal cyst which may arise from the pilosebaceous apparatus or eccrine sweat ducts. Histologically, the small cysts are lined by several layers of stratified squamous epithelium with central keratinous material, resembling a small epidermal cyst. They may be connected to a vellus hair follicle or eccrine sweat duct.

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A 28-year-old woman was evaluated for a 6-year history of multiple, asymptomatic papules located on the chest. Clinical examination revealed smooth surfaced, slightly erythematous, bluish papules on the chest (Fig. 1). The patient had noted gradual progression of the lesions over time on the same area. She had no notable past or family history. The histological examination revealed three round, well defined cystic structures on the upper and lower dermis (Fig. 2A). The first cystic lesion was com­posed of several squamous and granular layers. The cyst was filled with horny material arranged in laminated layers. These findings were consistent with milia (Fig. 2B). Read More…

INTRODUCTION

It is well established that each of the three parts of the hair follicle, namely, infundibulum, isthmus and the inferior portion originate from different types of cuteneous cyst. Especially Milia and EVHC can be considered as a subtype of a multiple pilosebaceous cyst and apocrine hidrocystoma is regarded as an adenomatous cystic proliferation of the apocrine glands. Read More…