COMP-angiopoietin 1 Gene Transfer Enhances Cutaneous Wound Healing: RESULTS
Ade-COMP-Ang 1 enhances the rate of wound healing
Analysis of the clinical wound healing rate in Ade-COMP-Ang 1-treated and Ade-LacZ-treated groups was performed via digital processing at 0, 3, 7 and 14 days after the wounding. The Ade- COMP-Ang 1-treated group showed 39.5%, 59.3%, and 91.8% reduction of the wound area at day 3, 7, and 14, respectively. On the other hand, the results in the Ade-LacZ-treated group revealed 20.4%, 46.2% and 89.0% reduction of the wound area, respectively. The differences in the results between the two groups at day 3 and 7 were statistically significant (p<0.05), however, the differences in the results at day 14 failed to show a statistical significance (Fig. 1).
Fig. 1. (A) The effect of Ade-COMP-Ang 1 on clinical wound healing. The treatment of Ade-COMP-Ang 1 showed 39.5%, 59.3% and 91.8% reduction of wound size at day 3, 7 and 14, compared with 20.4%, 46.2% and 89.0%, in the Ade-LacZ treated group, respectively. The results of day 3 and 7 between the Ade-COMP-Ang 1 treated group and the Ade-LacZ treated group were statistically significant (*p<0.05). (B) A representative example of the cutaneous wound healing of the Ade-COMP-Ang 1-treated and Ade-LacZ treated groups at day 0, 3, 7 and 14. The wound healing rate was accelerated in the Ade-COMP-Ang 1-treated group, compared with Ade-LacZ-treated group. Ade-LacZ-treated group: a (day 0), b (day 3), c (day 7), d (day 14 after wound surgery), Ade-COMP-Ang 1-treated group: e (day 0), f (day 3), g (day 7), h (day 14 after wound surgery). kamagra soft tabs
Ade-COMP-Ang 1 promotes the formation of new vessels
In order to investigate whether the Ade-COMP- Ang 1-induced-acceleration of wound healing was associated with an angiogenic effect, the number of vessels of the samples was evaluated by two independent investigators under a high power view (x200) at 3, 7, and 14 days after wound surgery. The mean vessel counts per specimen were averaged to give an overall mean vessel count per high-power field for each wound section. The number of blood vessels of the Ade-COMP-Ang 1-treated group was 73.7, 94.1, and 62.7 at day 3, 7, and 14, compared with the Ade-LacZ-treated group, i.e. those number of blood vessels was 53.5, 83.9 and 56.9, respectively. There were significant differences in the results at day 3, 7 and 14 between the two groups (p<0.05) (Fig. 2).
Fig. 2. (A) The effect of Ade-COMP-Ang 1 on angiogenesis. Under the high power field view (x200), the number of blood vessels of the Ade-COMP-Ang 1-treated group was 73.7, 94.1 and 62.7 at day 3, 7 and 14, respectively, compared with that of the Ade-LacZ treated group, i.e., 53.5, 83.9, and 56.9, respectively. The results of day 3, 7 and 14 between the Ade-COMP-Ang 1 treated group and the Ade-LacZ treated group were statistically significant (*p<0.05). (B) The blood vessel formation of the Ade-COMP-Ang 1 treated and Ade-LacZ treated groups at day 3, 7 and 14 (PECAM, x200). The number of blood vessels were increased in the the Ade-COMP-Ang 1-treated group, compared with Ade-LacZ-treated group. Ade-LacZ-treated group: a (day 3), c (day 7), e (day 14 after wound surgery), Ade-COMP-Ang 1-treated group: b (day 3), d (day 7), f (day 14 after wound surgery). levitra professional
